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微信小章| 产品名称： | NCTC clone 1469 derivative of NCTC 721 |
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| 商品货号： | TS211230 |
| Organism： | Mus musculus, mouse |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | newborn |
| Gender： | male |
| Strain： | C3H/An |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 41; range = 38 to 86. Two marker chromosomes present: One metacentric with one pair of arms having achromatic gaps near centromere; and an acrocentric with satellites. |
| Antigen Expression： | H-2k |
| Virus Susceptibility： | Vesicular stomatitis virus
Human poliovirus 1 |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
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| Subculturing： | Subcultures are prepared by scraping. Remove old medium, add fresh medium and dislodge the cells from the floor of the flask.xa0Aspirate cells and dispense the suspension into new flasks. A standard trypsinizaton may be used if desired. The culture medium in heavy cultures may be turbid in appearance and and give the gross impression of bacterial contamination. It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | VJ Evans |
| Deposited As： | Mus musculus |
| Year of Origin： | April, 1952 |
| References： | Hobbs GL, et al. Establishment of a clone of mouse liver cells from a single isolated cell. J. Natl. Cancer Inst. 18: 701-707, 1957. PubMed: 13502690 Evans VJ, et al. The growth in vitro of massive cultures of liver cells. J. Natl. Cancer Inst. 12: 1245-1265, 1952. PubMed: 14939026 J. Natl. Cancer Inst. 23: 823, 1959. J. Natl. Cancer Inst. 27: 29, 1961. Evans VJ, et al. Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture. Cancer Res. 12: 261-266, 1958. PubMed: 13523589 . . Fed. Proc. 17: 967, 1958. Westfall BB. Characterization of cells in tissue culture. Natl. Cancer Inst. Monogr. 7: 147-158, 1962. PubMed: 14006338 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |