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微信小章| 产品名称： | NCTC clone 2555 |
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| 商品货号： | TS211231 |
| Organism： | Mus musculus, mouse |
| Tissue： | subcutaneous connective tissue; areolar and adipose |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 82 days |
| Gender： | male |
| Strain： | C3H/HeN |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Karyotype： | model number = 56; range = 53 to 116. Bi-armed (metacentric and submetacentric) chromosomes noted in 50/50 cells. |
| Clinical Data： | male |
| Antigen Expression： | H-2k |
| Tumorigenic： | Yes, in irradiated C3H/HeN mice. |
| Virus Susceptibility： | Vesicular stomatitis virus
Human poliovirus 3 |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
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| Subculturing： | Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; CO2, 5% |
| Name of Depositor： | KK Sanford |
| Deposited As： | Mus musculus |
| Year of Origin： | September, 1956 |
| References： | Sanford KK, et al. Clonal analysis of variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 23: 1035-1059, 1959. PubMed: 14441477 Likely GD, et al. Further studies on the proliferation in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 13: 177-184, 1952. PubMed: 14946507 Sanford KK, et al. The development of variations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcoma-producing cells in vitro. J. Natl. Cancer Inst. 15: 215-237, 1954. PubMed: 13233880 Sanford KK. Clonal studies on normal cells and on their neoplastic transformation in vitro. Cancer Res. 18: 747-752, 1958. PubMed: 13573337 Sanford KK, et al. Studies on the difference in sarcoma-producing capacity of two lines of mouse cells derived in vitro from one cell. J. Natl. Cancer Inst. 20: 121-145, 1958. PubMed: 13502776 Scott DB, et al. Analysis of enzymatic activities of clones derived from variant cell lines transformed to malignant cells in tissue culture. J. Natl. Cancer Inst. 25: 1365-1379, 1960. PubMed: 13749340 Dupree, L. T. , et al. Influence of serum protein on determination of nutritional requirements of cells in culture, Exp. Cell Res., 28, 381 (1962) J. Natl. Cancer Inst. 23: 1059, 1960. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |