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微信小章| 产品名称： | NCTC 3749 |
|---|---|
| 商品货号： | TS211232 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | DBA/2 |
| Applications： | When the cells are grown in chemically defined medium (RPMI 1640 medium without serum), they produce IL-1 in response to stimulation with phorbol myristic acid (PMA) and lipopolysaccharide (LPS). Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line is a derivative of P388D1 (ATCC CCL-46). |
| Antigen Expression： | H-2d |
| Genes Expressed： | produce interleukin-1 (IL-1, interleukin 1),H-2d |
| Cellular Products： | produce interleukin-1 (IL-1, interleukin 1) |
| Virus Resistance： | poliovirus 1 |
| Comments： | This line is a derivative of P388D1 (ATCC CCL-46). When the cells are grown in chemically defined medium (RPMI 1640 medium without serum), they produce IL-1 in response to stimulation with phorbol myristic acid (PMA) and lipopolysaccharide (LPS). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | NCTC 135 medium, 100%
|
| Subculturing： | Subcultures are prepared by scraping. Remove one-half of the old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subcultivation Ratio: A ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: 5% Carbon dioxide (CO2) |
| Name of Depositor： | VJ Evans |
| Deposited As： | Mus musculus |
| References： | Mizel SB, et al. Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line P388D1. I. Enhancement of LAF production by activated T lymphocytes. J. Immunol. 120: 1497-1503, 1978. PubMed: 149171 Evans VJ, et al. A new tissue culture isolation and explanation of the P388 lymphocytic neoplasm in a chemically characterized medium. Exp. Cell Res. 32: 212-217, 1963. PubMed: 14094040 Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231 Dawe CJ, Potter M. Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro. Am. J. Pathol. 33: 603, 1957 Rabson AS, Leggallais FY. Cytopathogenic effect produced by polyoma virus in cultures of milk-adapted murine lymphoma cells (strain P388 D1). Proc. Soc. Exp. Biol. Med. 100: 229-233, 1959. PubMed: 13634094 . . Nature 181: 1343, 1958. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |