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微信小章| 产品名称： | NE |
|---|---|
| 商品货号： | TS211237 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | nullipotent embryonal carcinoma; teratocarcinoma |
| Age： | embryo; 7 days gestation |
| Strain： | C3H/J |
| Applications： | This nullipotent stem cell line does not differentiate either spontaneously or in response to retinoic acid. They secrete osteonectin, entactin, and fibronectin which bind to protein A and the 400000 and 200000 soluble laminins, but they do not form a visible extracellular matrix. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | osteonectin; entactin; fibronectin; laminin |
| Cellular Products： | osteonectin; entactin; fibronectin; laminin |
| Tumorigenic： | Yes |
| Effects： | Yes, when injected intramuscularly into syngeneic mice |
| Comments： | This nullipotent stem cell line does not differentiate either spontaneously or in response to retinoic acid. They express SSEA-1 antigen and alkaline phosphatase on the cell surface. The cells have a cytoskeleton rich in vimentin. They secrete osteonectin, entactin, and fibronectin which bind to protein A and the 400000 and 200000 soluble laminins, but they do not form a visible extracellular matrix. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:5 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | I Damjanov |
| Deposited As： | Mus musculus |
| References： | Damjanov A, et al. Basement membrane components secreted by mouse yolk sac carcinoma cell lines. Differentiation 45: 84-95, 1990. PubMed: 2098280 Gatalica Z, Damjanov I. Effects of alcohols on mouse embryonal carcinoma-substrate adhesion. Histochemistry 95: 189-193, 1990. PubMed: 2081693 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |