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微信小章| 产品名称： | NCI-H820 H820 |
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| 商品货号： | TS211238 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: lymph node |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | papillary adenocarcinoma |
| Age： | 53 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Near triploid; modal number = 69; range = 46 to 74; there were 25 to 35 marker chromosomes per metaphase; the i(7p), t(6p;??) and ?i(8q) were the few identifiable markers. One B-like chromosome (Bq+) had a length comparable to N1; another B size chromosome had an interstitial HSR segment. There were 1 to 2 structurally normal X chromosomes, and two or more Y chromosomes were detected in the QM stained metaphases. |
| Images： |  |
| Clinical Data： | 53 years Caucasian male |
| Tumorigenic： | YES |
| Effects： | in nude mice (The cells form typical papillary adenomas.) |
| Comments： | The cell line expresses three surfactant associated proteins (SP-A constitutively, and SP-B and SP-C after dexamethasone induction).xa0 Electron microscopy shows intracytoplasmic multilamellar bodies suggestive of Type II pneumocytes. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12 D16S539: 9,11 D5S818: 9,11 D7S820: 10,13 THO1: 8 TPOX: 12 vWA: 18 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1984 |
| References： | Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953 The NCI-H820 cell line was derived by A.F. Gazdar and H. Oie in 1984 from the lymph node of a patient with papillary adenocarcinoma of the lung taken prior to treatment. The cells form typical papillary adenomas. |