宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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NCI-H847 [H847]

货号 TS211244
中文名称 null
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产品名称: NCI-H847 H847
商品货号: TS211244
Organism: Homo sapiens, human
Tissue: lung; Derived from metastatic site: pleural effusion
Product Format: frozen
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage L,carcinoma; classic small cell lung cancer
Age: 63 years
Gender: male
Ethnicity: Caucasian, White
Applications:
The line was established in October 1984.
Derivation:
The line was established in October 1984.
Clinical Data:
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
male
Caucasian, White
63 years
Comments:
The line was established in October 1984.
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
40.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium.
Cells grow as floating aggregates of round cell clusters.
Cryopreservation:
Complete culture medium, 90%; additional fetal bovine serum, 5%; DMSO, 5%.
Culture Conditions:
Temperature: 37.0°C
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1984
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.