| 产品名称: | NCI-H865 H865 |
|---|---|
| 商品货号: | TS211245 |
| Organism: | Homo sapiens, human |
| Tissue: | lung |
| Product Format: | frozen |
| Culture Properties: | suspension, Cultures grow as floating aggregates of round cell clusters. |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease: | stage L,carcinoma; classic small cell lung cancer |
| Age: | 54 years |
| Gender: | female |
| Ethnicity: | Caucasian |
| Applications: | The line was established in November 1984. |
| Derivation: | The line was established in November 1984. |
| Clinical Data: | The patient received prior chemotherapy and radiation therapy. The patient was a smoker. female Caucasian 54 years |
| Comments: | The line was established in November 1984. The patient received prior chemotherapy and radiation therapy. The patient was a smoker. 40. |
| Complete Growth Medium: | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing: | Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) Cultures can be maintained by addition of fresh medium. |
| Cryopreservation: | Culture medium, 95%; DMSO, 5% |
| Culture Conditions: | Temperature: 37.0°C |
| STR Profile: | Amelogenin: X CSF1PO: 14 D13S317: 8 D16S539: 9 D5S818: 12 D7S820: 10 THO1: 9.3 TPOX: 8 vWA: 17 |
| Name of Depositor: | AF Gazdar, JD Minna |
| Deposited As: | Homo sapiens |
| Year of Origin: | 1984 |
| References: | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |