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微信小章| 产品名称： | NCI-H929 H929 |
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| 商品货号： | TS211247 |
| Organism： | Homo sapiens, human |
| Tissue： | bone marrow |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Age： | 62 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype： | Near tetraploid. Most copies of chromosome 8 have the 8q+ abnormality. |
| Images： |  |
| Derivation： | This cell line was established from a malignant effusion in a patient with myeloma. |
| Clinical Data： | 62 years Caucasian female This cell line was established from a malignant effusion in a patient with myeloma. |
| Antigen Expression： | PCA-1; CD38 + |
| Receptor Expression： | transferrin |
| Genes Expressed： | PCA-1; CD38 + |
| Comments： | The cells are positive for plasma cell antigen 1 (PCA-1), transferrin receptor, CD38 (T10), but are negative for HLA DR, CALLA and markers of early B cell development. They are negative for Epstein-Barr virus nuclear antigen (EBNA). The cells have a rearrangement of the c-myc proto oncogene and express c-myc RNA. There is also an activated ras allele. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 X 105 viable cells/mL.xa0Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Interval: Maintain cultures at cell concentrations between 5 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 9,13 D5S818: 11,12 D7S820: 10,12 THO1: 9.3 TPOX: 8,11 vWA: 14,15 |
| Isotype： | IgA kappa (Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.) |
| Name of Depositor： | National Cancer Institute |
| Deposited As： | Homo sapiens |
| U.S. Patent Number： | |
| References： | Hollis GF, et al. Complex translocation disrupts c-myc regulation in a human plasma cell myeloma. Mol. Cell. Biol. 8: 124-129, 1988. PubMed: 3275865 Gazdar AF, et al. Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood 67: 1542-1549, 1986. PubMed: 2423157 Ernst TJ, et al. Identification of a second transforming gene, rasn, in a human multiple myeloma line with a rearranged c-myc allele. Blood 72: 1163-1167, 1988. PubMed: 3048435 Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain. Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain. |