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微信小章| 产品名称： | NCI-H727 H727 |
|---|---|
| 商品货号： | TS211250 |
| Organism： | Homo sapiens, human |
| Tissue： | lung, bronchus |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoid |
| Age： | 65 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from tissue taken prior to therapy. |
| Clinical Data： | 65 years
Caucasian
female
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from tissue taken prior to therapy. |
| Receptor Expression： | epidermal growth factor (EGF) |
| Genes Expressed： | neuromedin B (NMB) |
| Cellular Products： | neuromedin B (NMB) |
| Comments： | This is the best differentiated of the available bronchial carcinoid lines.
The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung.
The cells are able to synthesize the peptide NMB (at 0.1 pmol/mg protein), but not the gastrin releasing peptide (GRP).
The cell line secretes a parathyroid hormone-like protein which is calcium stimulated through a protein kinase C pathway.
Growth of NCI-H727 cells is inhibited by epidermal growth factor (EGF) receptor monoclonal antibodies. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | div>Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 11 D16S539: 11,13 D5S818: 11,12 D7S820: 8,10 THO1: 8 TPOX: 8 vWA: 14,15 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| References： | Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 Brandt DW, et al. Calcium-stimulated parathyroid hormone-like protein secretion: potentiation through a protein kinase-C pathway. Endocrinology 128: 2999-3004, 1991. PubMed: 2036974 Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Natl. Cancer Inst. Monogr. 13: 117-123, 1992. |