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| 产品名称： | NCI-H735 H735 |
|---|---|
| 商品货号： | TS211251 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: liver |
| Product Format： | frozen |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E, carcinoma; small cell lung cancer |
| Age： | 58 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established in August 1984. |
| Clinical Data： | The patient received prior chemotherapy. The patient was a smoker. female Caucasian 58 years |
| Complete Growth Medium： | HITES medium (serum free) HITES medium is formulated at the ATCC as follows: Dulbeccos medium : Hams F12, 50:50 mix (ATCC 30-2006) Insulin 0.005 mg/ml Transferrin 0.01 mg/ml Sodium selenite 30 nM Hydrocortisone 10 nM beta-estradiol 10 nM HEPES 10 mM L-glutamine 2 mM (in addition to that in the base medium)
|
| Cryopreservation： | Freeze Medium:xa0Complete growth medium supplemented with 5% FBS and 5% (v/v) DMSO
Storage Temperature:xa0Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: 5% CO2 in air. |
| STR Profile： | Amelogenin: X CSF1PO: 12,14 D13S317: 11,14 D16S539: 14 D5S818: 9,11 D7S820: 8,11 THO1: 8,9.3 TPOX: 11 vWA: 18,19 |
| Year of Origin： | 1984 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |