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微信小章| 产品名称： | NCI-H740 H740 |
|---|---|
| 商品货号： | TS211254 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: lymph node |
| Product Format： | frozen |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E, carcinoma; classic small cell lung cancer |
| Age： | 63 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established in June 1984. |
| Clinical Data： | 63 years
male
Caucasian
The patient was a smoker. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | CRL-5840 cells grow as aggregates in suspension.xa0 Cultures may lose viability when the aggregates are dispersed.xa0 Cultures can be maintained by the addition of fresh medium. A split ratio of 1:2 to 1:4 should be used and the cell line is subcultured when there are numerous healthy appearing clusters present in suspension. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC 4-X. |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 9,12 D13S317: 11,14 D16S539: 12 D5S818: 12 D7S820: 9,10 THO1: 6,9 TPOX: 8,10 vWA: 15,16 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1984 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |