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微信小章| 产品名称： | NCI-H774 H774 |
|---|---|
| 商品货号： | TS211255 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: soft tissue |
| Product Format： | frozen |
| Morphology： | floating aggregates of round cell clusters |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E, carcinoma; classic small cell lung cancer |
| Age： | 43 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | del(p13-p21), del(p11-pter), del(p13-pter) |
| Derivation： | The line was established in July 1984. |
| Clinical Data： | The patient was a smoker male Caucasian 43 years old 30 pack years |
| Comments： | Fast growing.
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| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters. Single cells are dead and clusters are viable. Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density). |
| Cryopreservation： | Culture medium, 90%; DMSO, 5%; extra FBS, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 8 D16S539: 12 D5S818: 11 D7S820: 9,11 THO1: 6,9.3 TPOX: 8 vWA: 15,17 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1984 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |