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| 产品名称： | NCI-H747 H747 |
|---|---|
| 商品货号： | TS211256 |
| Organism： | Homo sapiens, human |
| Tissue： | cecum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent, floating aggregates of round cells with some attached cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | colorectal adenocarcinoma |
| Age： | 69 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 66; range = 63 to 75 This is a hypotriploid human cell line. The modal chromosome number is 66, occurring in 20% of cells. The rate of higher ploidies is 1.6%. Twelve marker chromosomes are common to all cells; these include single copy for i(1q), i(7p), i(10q); two copies for i(3q) and two to three copies for der(12)t(12;13)(q24,33;q12). The N12, N13, X and Y have single copy each. The presence of the Y was confirmed by Q-banding. |
| Derivation： | This line was derived from a metastasis in a common duct node from a patient prior to therapy. |
| Clinical Data： | 69 years
Caucasian
male |
| Antigen Expression： | Blood type A; Rh+; CA19-9 antigen |
| Genes Expressed： | carcinoembryonic antigen (CEA), 8 ng/mL per 106 cells per 10 days |
| Cellular Products： | carcinoembryonic antigen (CEA), 8 ng/ml per 10 exp6 cells per 10 days |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice Tumors developed in 3 of 5 nude mice inoculated subcutaneously with 107 cells xa0 |
| Comments： | The cells are negative for Dopa decarboxylase and TAG-72 antigen but are positive for CEA and CA19-9 antigen. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor： | AF Gazdar |
| Deposited As： | Homo sapiens |
| References： | Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249 |