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微信小章| 产品名称： | NCI-H650 H650 |
|---|---|
| 商品货号： | TS211259 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | spherical |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | bronchioalveolar carcinoma; non-small cell lung cancer |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Karyotype： | modal number = 90; range = 54 to 178; del(p14-p23), inv(3p) |
| Images： |  |
| Clinical Data： | The tissue donor was a non-smoker. |
| Complete Growth Medium： | The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%. |
| Subculturing： | Cultures can be maintained by the addition of fresh medium. Cells grow as floating aggregates of round cell clusters. Medium Renewal: Add fresh medium every 3 to 4 days. |
| Cryopreservation： | Complete growth medium, 92.5%; DMSO, 7.5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 8,12 D16S539: 8,11 D5S818: 11 D7S820: 10,11 THO1: 6,9.3 TPOX: 8,11 vWA: 17 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | October, 1983 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |