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微信小章| 产品名称： | NCI-H510A H510A, NCI-H510 |
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| 商品货号： | TS211275 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic tissue: adrenal gland |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma; small cell lung cancer; extrapulmonary origin |
| Age： | 56 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | hypotriploid; modal number = 54; range = 46 to 57. Twenty to 25 marker chromosomes were common to all cells. These included t(13q21q), der(1)t(1;21)(p36.1;q11), der(7)t(7;7)(p22;q22), 11p+, 12p+, and 15p+. Neither DM nor HSR were detected; structurally normal N1, N2, N13 and N21 were absent. Generally, there were 3 copies of both F group chromosomes; the X chromosomes were paired, and structurally normal Y chromosome was not found. |
| Images： |  |
| Derivation： | The NCI-H510A cell line was derived by D. Carney, A.F. Gazdar and associates in 1982 from an adrenal metastasis in an adult male patient. |
| Clinical Data： | 56 years
Caucasian
male
|
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (The cells form transplantable tumors with typical small cell carcinoma histology.) |
| Comments： | The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue.xa0 The line does not exhibit any gross structural DNA abnormalities.xa0 The cells express elevated levels of four biochemical markers of SCLC: neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. The cells form transplantable tumors with typical small cell carcinoma histology. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | This line grows as a mixture of cells in suspension and adherent cells. Subcultures can be prepared by scraping the adherent cells into the medium, collecting the cells by centrifugation, resuspending in fresh medium and dispensing into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: culture medium, 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 11,12 D16S539: 13 D5S818: 9,12 THO1: 7,9.3 TPOX: 8 vWA: 15 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1982 |
| References： | Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257 |