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微信小章| 产品名称： | NCI-H508 H508 |
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| 商品货号： | TS211276 |
| Organism： | Homo sapiens, human |
| Tissue： | cecum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | floating aggregates of round cells with some attached cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | colorectal adenocarcinoma |
| Age： | 55 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Karyotype： | modal number = 102; range = 71 to 131; most cells have DMs |
| Derivation： | This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. |
| Clinical Data： | 55 years Caucasian male |
| Antigen Expression： | Blood type A; Rh+; CA19-9 antigen |
| Genes Expressed： | carcinoembryonic antigen (CEA), 1709 ng/mL per 106 cells per 10 days |
| Cellular Products： | carcinoembryonic antigen (CEA), 1709 ng/mL per 106 cells per 10 days |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells xa0 |
| Comments： | The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 8,12 D16S539: 12 D5S818: 12 D7S820: 8,12 THO1: 9,9.3 TPOX: 8,11 vWA: 15,16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor： | AF Gazdar |
| Deposited As： | Homo sapiens |
| References： | Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249 |