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| 产品名称： | NCI-H498 H498 |
|---|---|
| 商品货号： | TS211277 |
| Organism： | Homo sapiens, human |
| Tissue： | cecum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | suspension, with some loosely adherent cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | colorectal adenocarcinoma |
| Age： | 56 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy. The cells form glandular structures and secrete mucin. The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA. Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 51; range = 47 to 54. This is a hyperdiploid human cell line. The modal chromosome number is 51, occurring in 42% of the cells. The rate of higher ploidies is 5%. The 2q+ is the only marker chromosome detected and common to all cells. There are four copies for N7; 3 copies each for N8, N13 and N22, and a single copy for the X. |
| Derivation： | This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy. Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity. |
| Clinical Data： | This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy. Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity. male Caucasian 56 years |
| Antigen Expression： | Rh+; CA19-9 antigen; TAG-72 antigen |
| Genes Expressed： | This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. carcinoembryonic antigen (CEA), 826 ng/ml per 10 exp6 cells per 10 days; mucin,Rh+; CA19-9 antigen; TAG-72 antigen,The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA. |
| Cellular Products： | carcinoembryonic antigen (CEA), 826 ng/ml per 10 exp6 cells per 10 days; mucin |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy. The cells form glandular structures and secrete mucin. The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA. Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium.
Alternatively, the cells may be collected by centrifugation and then resuspended at 2 to 4 X 10(4) viable cells/ml. When the cell concentration becomes heavy with large clumps, aspirate well and and dilute approximately 1:10.
If the clumps are allowed to get too large, most of the cells will die. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 9,11 D16S539: 9,11 D5S818: 13 D7S820: 8,11 THO1: 7 TPOX: 8 vWA: 15 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | AF Gazdar |
| Deposited As： | Homo sapiens |
| References： | Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249 Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345 Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells |