宁波泰斯拓生物

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NCI-H2171 [H2171]

货号 TS211288
中文名称 null
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产品名称: NCI-H2171 H2171
商品货号: TS211288
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: pleural effusion
Cell Type: lymphoblast
Product Format: frozen
Morphology: floating clusters
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E, carcinoma; small cell lung cancer
Age: 50 years
Gender: male
Ethnicity: Caucasian
Karyotype: iso(3q)
Derivation:

The line was established in April 1989 from a pleural effusion metastasis of a lung.

Clinical Data:
50 years
Caucasian
male
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
150 pack years
Comments:

A lymphoblastoid line from the same patient is available as ATCC CRL-5969.

Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Cryopreservation:
Culture medium, 95%; DMSO, 5%
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1989
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.