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微信小章| 产品名称： | NCI-H2141 H2141 |
|---|---|
| 商品货号： | TS211289 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: lymph node |
| Cell Type： | lymphoblast |
| Product Format： | frozen |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E, carcinoma; small cell lung cancer |
| Age： | 58 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | 3p is normal |
| Derivation： | The line was established in March 1989 from lymph node, a metastatic site.xa0 A lymphoblastoid line from the same patient is available as ATCC CRL-5955. |
| Clinical Data： |
The patient received prior chemotherapy and radiation therapy. The patient was a smoker. 50 pack years |
| Comments： | A lymphoblastoid line from the same patient is available as ATCC CRL-5955. |
| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters. Single cells are dead and clusters are viable. Medium Renewal: Add fresh medium as cell density increases. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Atmosphere: 5% CO2 in airxa0 |
| STR Profile： | D5S818: 12 D13S317: 14 D7S820: 10, 12 D16S539: 8 vWA: 19 TH01: 6, 8 TPOX: 8, 9 Amelogenin: X Y CSF1PO: 11 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1989 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |