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| 产品名称： | NCI-H2195 H2195 |
|---|---|
| 商品货号： | TS211292 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metatstatic site: bone marrow |
| Cell Type： | lymphoblast |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E, carcinoma; small cell lung cancer |
| Age： | 67 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established in June 1989 from bone marrow metastasis of a lung. |
| Clinical Data： | 67 yearsxa0
Caucasian
male |
| Comments： | A lymphoblastoid line from the same patient is available as ATCC CRL-5956. |
| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
|
| Subculturing： |
Subcultivation Ratio: A ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice weekly |
| Cryopreservation： | Freeze medium: Culture medium, 90%; DMSO, 5%; FBS ,5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11 D13S317: 11 D16S539: 10 D5S818: 12 D7S820: 10,11 THO1: 9.3 TPOX: 8,9 vWA: 18 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1989 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. 100 pack years The line was established from tissue taken from a patient with a 100 pack-year history of smoking. |