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| 产品名称： | NCI-H2198 H2198 |
|---|---|
| 商品货号： | TS211293 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; Derived from metastatic site: lymph node |
| Product Format： | frozen |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E,carcinoma; small cell lung cancer |
| Age： | 67 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | The line was established in June 1989. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established in June 1989. |
| Clinical Data： | The patient was a smoker. male Caucasian 67 years |
| Comments： | The line was established in June 1989. The patient was a smoker. 100 pack years. |
| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation： | Freeze medium: 50% FBS + 40% Growth Medium + 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1989 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |