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微信小章| 产品名称： | NCI-H2227 H2227 |
|---|---|
| 商品货号： | TS211296 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Product Format： | frozen |
| Culture Properties： | mixed, adherent and suspension (floating clusters in suspension with some adherent cells) |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E, carcinoma; small cell lung cancer |
| Age： | 54 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | Patient was a smoker. 70 pack years.xa0 |
| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Attached cells can be scraped.
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Culture medium, 95%; additional fetal bovine serum, 5%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | August, 1989 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. floating clusters in suspension with some adherent cells |