微信小陈
微信小章
产品简介
购买须知
| 产品名称： | NCI-H2087 H2087 |
|---|---|
| 商品货号： | TS211300 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: lymph node |
| Product Format： | frozen |
| Morphology： | epithelial-like and/or rounded |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage 1, adenocarcinoma; non-small cell lung cancer |
| Age： | 69 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established in November 1988 from a lymph node metastasis of a lung adenocarcinoma. |
| Clinical Data： | 69 years
Caucasian
male
The patient was a smoker
Sixty packs year
|
| Comments： | A lymphoblastoid line from the same patient is available as ATCC® CRL-5965 (NCI-BL2087). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Add fresh medium twice weekly |
| Cryopreservation： | Freeze medium: RPMI 1640 medium, 85%; fetal bovine serum, 10%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D5S818: 11 D13S317: 12 D7S820: 8, 10 D16S539: 9, 11 THO1: 7, 9 TPOX: 11 vWA: 17 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | November, 1988 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |