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| 产品名称： | NCI-H2122 H2122 |
|---|---|
| 商品货号： | TS211305 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: pleural effusion |
| Cell Type： | lymphoblast |
| Product Format： | frozen |
| Morphology： | it is normal for CRL-5985 to show a mixed morphology of rounded cells and epithelial cells. This cell line produces moderate to large, viable, loose, grape-like clusters that either loosely adhere to the flask or remain in suspension |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage 4, adenocarcinoma; non-small cell lung cancer |
| Age： | 46 years |
| Gender： | female |
| Ethnicity： | Caucasian, White |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established in January 1989 from pleural effusion metastasis of lung. |
| Clinical Data： | 46 years Caucasian female The patient was a smoker 30 pack year |
| Comments： | A lymphoblastoid line from the same patient is available as ATCC CRL-5967.
|
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 10 D16S539: 9,12 D5S818: 11,12 D7S820: 8,10 THO1: 7,9.3 TPOX: 9 vWA: 17 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | January, 1989 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |