微信小陈
微信小章
产品简介
购买须知
| 产品名称： | NCI-H2135 H2135 |
|---|---|
| 商品货号： | TS211306 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Product Format： | frozen |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | non-small cell lung cancer |
| Applications： | The line was established in February 1989. |
| Derivation： | The line was established in February 1989. |
| Clinical Data： | The tissue donor was a non-smoker. |
| Comments： | The line was established in February 1989. The tissue donor was a non-smoker. |
| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
|
| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 2 X 10 exp5 and 5 X 10 exp5 viable cells/ml. Adherent cells may be harvested by scraping. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 11,12 D5S818: 12,13 D7S820: 11,12 THO1: 9.3 TPOX: 8 vWA: 17,18 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1989 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |