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| 产品名称： | NCI-H2126 H2126 |
|---|---|
| 商品货号： | TS211307 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma; non-small cell lung cancer |
| Age： | 65 years adult |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Modal number = 79; range = 71 to 83 This is a hypertriploid human cell line with the modal chromosome number of 79, occurring in 20% of cells. The rate of cells with a higher ploidy was 5.5%. Karyotypes were very complex. There were over 22 marker chromosomes commonly present in most cells, many with complex structural rearrangements. Among these markers were: double copies for t(10qter--10q11.2::?::13C--13qter) and der(9)t(3;9) (p12;p22?), and one copy each for del (1) (p22) and i (iq). There were two normal X chromosomes per cell. Normal chromosomes Y, N1, N5, N14, and N15 were not found. Chromosomes N20 and N22 generally had four or more copies per cell. |
| Clinical Data： | 65 years adult Caucasian male |
| HeLa Markers： | N |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within one month in 4/5 nude mice inoculated subcutaneously with 10(7) cells.) |
| Comments： | An EBV-transformed lymphoblastoid cell line from the same patient is available as TS211307.1. |
| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 12,14 D16S539: 12 D5S818: 11 D7S820: 8,9 THO1: 7,9.3 TPOX: 8 vWA: 17 |
| Isoenzymes： | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1-2 PGM3, 2 |
| Population Doubling Time： | about 41 hours |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |