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| 产品名称： | NCI-H2052 H2052 |
|---|---|
| 商品货号： | TS211316 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: pleural effusion |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage 4, mesothelioma |
| Age： | 65 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Derivation： | The line was established in September 1988.xa0Derived from metastatic site, pleural effusion |
| Clinical Data： | 65 years A lymphoblastoid line from the same patient is available as ATCC CRL-5963. Caucasian male The patient was a smoker. |
| Comments： | A lymphoblastoid line from the same patient is available as ATCC CRL-5963. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: 1:3 to 1:6 |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 12 D16S539: 11,13 D5S818: 11 D7S820: 12 THO1: 7,9.3 TPOX: 8,11 vWA: 17 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1988 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |