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| 产品名称： | NCI-H2073 H2073 |
|---|---|
| 商品货号： | TS211317 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage 3A, adenocarcinoma; non-small cell lung cancer |
| Age： | 47 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established in November 1988. |
| Clinical Data： | 47 years
Caucasian
female
The patient was a smoker.Thirty pack years |
| Comments： | ATCC® CRL-5909™ and CRL-5918™ arose from the same donor (see Mayba O, 2014) |
| Complete Growth Medium： | The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%. |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
|
| Cryopreservation： | Freeze medium: culture medium, 85%; FBS,10%; DMSO,5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 13 D13S317: 13 D16S539: 12 D5S818: 12 D7S820: 12 THO1: 6,9.3 TPOX: 9 vWA: 17 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1988 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Mayba O, et al. Integrative analysis of two cell lines derived from a non-small-lung cancer patient – A panomics approach. Pac Symp Biocomput 2014: 75-86. PubMed: 24297535 |