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微信小章| 产品名称： | NCI-H1694 H1694 |
|---|---|
| 商品货号： | TS211331 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | floating clusters |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E, carcinoma; classic small cell lung cancer |
| Age： | 61 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | del(p21-pter) |
| Derivation： | The line was established in July 1987 from ascites, a metastatic site. |
| Clinical Data： | The tissue donor was a non-smoker.
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| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of large clusters of cells. Some cells may attach.xa0 Medium Renewal: Add medium as cell density increases. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 9,11 D5S818: 12 D7S820: 11 THO1: 6,9 TPOX: 11 vWA: 15,16 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1987 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |