微信小陈
微信小章
产品简介
购买须知
| 产品名称： | NCI-H1703 H1703 |
|---|---|
| 商品货号： | TS211333 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Cell Type： | squamous cell |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lung; squamous cell; non-small cell lung cancer |
| Age： | 54 years adult |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | NCI-H1703 was derived from a stage 1 lung squamous cell carcinoma of a 54-year-old Caucasian male smoker. |
| Clinical Data： | 54 years adult Caucasian male The tissue donor was a smoker (50 pack years). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| STR Profile： | Amelogenin: X,Y
CSF1PO: 12
D13S317: 10
D16S539: 10,12
D5S818: 11
D7S820: 10,12
THO1: 7
TPOX: 8,11
vWA: 16,17
|
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | August, 1987 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |