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| 产品名称： | NCI-H1793 H1793 |
|---|---|
| 商品货号： | TS211335 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma; non-small cell lung cancer |
| Age： | 52 years |
| Gender： | female |
| Derivation： | The line was established in December 1987. |
| Clinical Data： | female The tissue donor was a non-smoker. |
| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended |
| Cryopreservation： | Freeze medium: RPMI 1640, 85%; fetal bovine serum, 10%; DMSO, 5%. |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 9,12 D13S317: 8,12 D16S539: 13 D5S818: 11,12 D7S820: 7,8 THO1: 9,9.3 TPOX: 8 vWA: 18 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1987 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |