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| 产品名称： | NCI-H1618 H1618 |
|---|---|
| 商品货号： | TS211339 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: bone marrow |
| Product Format： | frozen |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E,carcinoma; small cell lung cancer |
| Age： | 55 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established in April 1987. |
| Clinical Data： | Caucasian female The patient was a smoker. 50 pack years. |
| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
|
| Subculturing： | Cells can be maintained by the addition of fresh medium
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 9 D16S539: 11,13 D5S818: 10 D7S820: 8 THO1: 6 TPOX: 8,11 vWA: 16,17 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | April, 1987 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760 |