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| 产品名称： | NCI-H1672 H1672 |
|---|---|
| 商品货号： | TS211346 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Cell Type： | lymphoblast |
| Product Format： | frozen |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage L,carcinoma; classic small cell lung cancer |
| Age： | 58 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established in July 1987. |
| Clinical Data： | The patient was a smoker.
male
Caucasian
58 years |
| Comments： | A lymphoblastoid line from the same patient is available as ATCC CRL-5959. |
| Complete Growth Medium： | HITES medium supplemented with 5% fetal bovine serum
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium. Cells grow as floating aggregates of round cell clusters.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) |
| Cryopreservation： | Freeze medium:
Complete growth medium supplemented with an additional 15% fetal bovine serum and 5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11 D16S539: 11 D5S818: 11,13 D7S820: 10 THO1: 10 TPOX: 11 vWA: 17 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | July, 1987 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |