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微信小章| 产品名称： | NCI-H146 H146 |
|---|---|
| 商品货号： | TS211356 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: bone marrow |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma; small cell lung cancer |
| Age： | 59 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. At least 15 markers were common to all cells including single copies of der(1)t(1;13)(p32;q12) and del(3)(p21/22), and paired der(7)t(7;?)(p22;?), t(13q16q) and t(16p21q). Ten others were seen in single copies or paired. No HSR or DM were found. Structurally normal N5, N13 and N14 were absent. Four or more copies of N18 and N20 were present in each cell, and N5, N7 and N11 were mostly single copies per cell. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations. |
| Images： |  |
| Clinical Data： | 59 years Caucasian male |
| Receptor Expression： | insulin-like growth factor II (IGF II) |
| Oncogene： | myc +; myb +; raf +; ras +; fms +; fes - |
| Genes Expressed： | The cells do not express vasopressin, oxytocin or gastrin releasing peptide.
The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein. There is expression of c-myb, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs. Oncogenes: myc +; myb +; raf +; ras +; fms +; fes - |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified.
There is expression of c-myb, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs.
The line expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | The line should be subcultured by dilution with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 11,12 D16S539: 11 D5S818: 12 D7S820: 9,10 THO1: 6,9.3 TPOX: 8,11 vWA: 14,16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 2 PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1979 |
| References： | Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917 Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141 Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257 Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028 Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370 Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893 Lung Cancer 4: 155-161, 1988. The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from a bone marrow metastasis of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy. The cells form transplantable tumors which histologically resemble tumor cells from the original biopsy specimen. |