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微信小章| 产品名称： | NCI-H128 H128 |
|---|---|
| 商品货号： | TS211363 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | floating aggregates |
| Culture Properties： | floating aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma; small cell lung cancer |
| Age： | 60 years |
| Gender： | male |
| Ethnicity： | Black |
| Applications： | It retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. |
| Karyotype： | There are 2 distinct aneuploid peaks, both have characteristic 3p deletion. |
| Clinical Data： | 60 years Black male |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | This cell line is aneuploid. Will form colonies in soft agar. It retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. NCI-H128 cells do well on a rotary shaker flask at 70 to 80 rpm at 37°C. It is normal for cultures of this line to have fairly large amounts of cell debris. |
| Complete Growth Medium： | Iscoves Modified Dulbeccos Medium (ATCC® 30-2005), 80%; fetal bovine serum (ATCC® 30-2020), 20% |
| Subculturing： | Allow cell aggregates to settle to the bottom of the flask, discard the supernatant medium, disperse the cells with gentle pipetting and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommendedMedium Renewal: Twice per week |
| Cryopreservation： | Culture medium, 90%; DMSO, 10% |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 12 D16S539: 13 D5S818: 9 D7S820: 8,10 THO1: 6,9 TPOX: 8 vWA: 17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, A GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor： | AF Gazdar |
| Deposited As： | Homo sapiens |
| References： | Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201 Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156 Adi F, et al. Establishment of Continuous, Clonable Cultures of Small-Cell Carcinoma of the Lung Which Have Amine Precursor Uptake and Decarboxylation Cell Properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156 Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257 Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258 Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370 Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760 The cells form tumors with typical small cell carcinoma histology. |
| Cross References： | Nucleotide (GenBank) : M22491 Human bone morphogenetic protein-3 (BMP-3) mRNA. |