微信小陈
微信小章
产品简介
购买须知
| 产品名称： | NCI-BL2347 BL2347 |
|---|---|
| 商品货号： | TS211376 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast; Epstein-Barr virus (EBV) transforme |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells contain Epstein-Barr Virus (EBV) a Herpesvirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 54 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Clinical Data： | The tumor cell line from this patient is available as ATCC CRL-5942. female Caucasian 54 years |
| Comments： | The tumor cell line from this patient is available as ATCC CRL-5942. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by addition of fresh medium or
replacement of medium. Alternatively, cultures can be
established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 x 10 exp5 viable cells/ml. Maintain cultures at a cell concentration between 3 X 10 exp5 and 2 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 12,14 D16S539: 11,12 D5S818: 11 D7S820: 10,11 THO1: 9.3 TPOX: 8 vWA: 16,19 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |