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| 产品名称： | NCI-BL1339 BL1339, NCI-BL6 |
|---|---|
| 商品货号： | TS211382 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast; Epstein-Barr virus (EBV) transforme |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 xa0Cells contain Epstein-Barr Virus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 49 years |
| Gender： | female |
| Ethnicity： | Caucasian, White |
| Clinical Data： | female
Caucasian, White
49 years |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp6 and 3 X 10 exp6 viable cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11,13 D5S818: 11,13 D7S820: 10,12 THO1: 7,9.3 TPOX: 8,11 vWA: 17,19 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |