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微信小章| 产品名称： | NAMALWA |
|---|---|
| 商品货号： | TS211391 |
| Organism： | Homo sapiens, human |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells contain Herpesvirus Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Age： | 3 years |
| Gender： | female |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a human cell line with the hypodiploid chromosome count. The modal chromosome number was 44, occurring in 30% of cells. The rate of cells with higher ploidies was 2.8%. Twelve to 14 marker chromosomes were common to most cells. Among these were ins(1;?) (q11;?), ins(3) (q11q29), del(3) (p12/13), der(6)t(3;6) (p21;p25), t(15q21q) and seven to nine others. Normal N3 was absent, and N7 had three copies per cell. The X chromosome was single. There was no Y chromosome. |
| Images： |  |
| Clinical Data： | female
3 years |
| Genes Expressed： | immunoglobulin |
| Comments： | The cells contain the Epstein-Barr virus (EBV) genome. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 92.5%; fetal bovine serum, 7.5%
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Cultures should be started at 5 x 105 viable cells/mL and subcultured at 2 x 106 cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 9 D5S818: 12,13 D7S820: 11 THO1: 7,9.3 TPOX: 6,11 vWA: 14 |
| Isotype： | IgM; lambda light chain |
| Name of Depositor： | NB Finter |
| Deposited As： | Homo sapiens |
| References： | Klein F, et al. Large-scale production and concentration of human lymphoid interferon. Antimicrob. Agents Chemother. 15: 420-427, 1979. PubMed: 464569 Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitts and non-Burkitts type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763 Klein G, et al. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int. J. Cancer 10: 44-57, 1972. PubMed: 4122458 Nyormoi O, et al. Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line. Int. J. Cancer 12: 396-408, 1973. PubMed: 4365093 |
| Cross References： | Nucleotide (GenBank) : X59969 H.sapiens BiP/GRP78 promoter. |