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微信小章| 产品名称： | NC-37 |
|---|---|
| 商品货号： | TS211396 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Gender： | male |
| Applications： | The consistency of the lines characteristics has made it a useful tool in a wide variety of biochemical, immunological, virological and cell biological studies such as being usedxa0as a target cell for EBV superinfection studies. It alsoxa0has proven useful in chemical induction studies of the latent EBV genome and in the propagation of various (retroviruses) oncornaviruses. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 48; range = 40 to 51 |
| Derivation： | Although deposited with the ATCC as a lymphoblastoid cell line established from a 34-year-old male caucasian, DNA fingerprinting has shown this line to be a derivative of Raji (see ATCC CCL-86), a Burkitts lymphoma cell line. |
| Comments： | Each cell contains an average of 60 EBV genome copies. The cells are EBNA positive (EBNA+) and surface immunoglobulin negative (sIg-). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Maintain cultures at 5xa0x 105 viable cells/mL. A maximum density of up to 2xa0x 106 is possible.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 3 to 4 days |
| Cryopreservation： | Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13,14 D16S539: 8,11 D5S818: 10,13 D7S820: 10 THO1: 6,7 TPOX: 8,13 vWA: 16 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | J Wolff |
| Deposited As： | Homo sapiens |
| Year of Origin： | February, 1967 |
| References： | Durr FE, et al. Studies on the infectivity and cytopathology of Epstein-Barr virus in human lymphoblastoid cells. Int. J. Cancer 6: 436-449, 1970. PubMed: 4321017 Mayyasi SA, et al. The coating reaction of the herpes-type virus isolated from malignant tissues with an antibody present in sera. Cancer Res. 27: 2020-2024, 1967. PubMed: 6073499 The NC-37 cell line was reportedly initiated by W. Korol from peripheral blood from a donor whose serum was positive for EBV antibodies as determined by immunofluorescence and virus coating test. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |