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微信小章| 产品名称： | MYA-1 |
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| 商品货号： | TS211400 |
| Organism： | Felis catus, cat |
| Tissue： | peripheral blood |
| Cell Type： | T lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pathogen-free |
| Age： | 5 months |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | MYA-1 is a T lymphoblastoid cell line that was established from blood mononuclear cells from a specific pathogen-free cat in 1988. |
| Antigen Expression： | feline CD3 +; feline CD4 +; feline CD8 - |
| Receptor Expression： | interleukin 2 (IL-2) |
| Comments： | The cells are interleukin-2 (Il-2) dependent.xa0They are positive for IL-2 receptor, feline CD3, feline CD4 and major histocompatibility complex (MHC) class II antigen.xa0They are negative for feline CD8. FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells. xa0Ref
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| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100 units/ml recombinant human IL-2 and 10% fetal bovine serum
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
xa0 |
| Cryopreservation： | culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | T Miyazawa |
| Deposited As： | cat |
| Year of Origin： | 1988 |
| References： | Miyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760 Miyazawa T, et al. Further characterization of a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive for feline immunodeficiency virus. J. Vet. Med. Sci. 54: 173-175, 1992. PubMed: 1313703 |
Miyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760