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| 产品名称： | N1-S1 |
|---|---|
| 商品货号： | TS211405 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | liver |
| Product Format： | frozen |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Hepatoma; Novikoff hepatoma |
| Gender： | male |
| Strain： | Sprague-Dawley |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The N1-S1 cell line was established from a hepatoma induced by feeding 4-dimethylaminoazobenzene to a male rat. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 104 viable cells/mL and subculture at 1 to 1.5 X 106 cells/mLxa0(after about 3 days). Excessive clumping of cells can often be cured by trypsinizing the cells to disperse them. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | JE Becker |