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微信小章| 产品名称： | N1E-115 |
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| 商品货号： | TS211406 |
| Organism： | Mus musculus, mouse |
| Tissue： | brain |
| Cell Type： | neuroblast |
| Product Format： | frozen |
| Morphology： | neuroblast |
| Culture Properties： | loosely adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Strain： | A/J |
| Applications： | This cell line is a suitable transfection host.xa0The cells can be used to study neurotransmitters and their receptors with radioligand binding, second messenger synthesis and electrophysiological changes. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 192 |
| Images： |  |
| Derivation： | The N1E-115 cell line was established in 1971 by T. Amano, E. Richelson, and M. Nirenberg by cloning the C-1300 spontaneous mouse neuroblastoma tumor, C-1300. |
| Receptor Expression： | acetylcholine, muscarinic m1; acetylcholine, muscarinic m2; vasoactive intestinal peptide (VIP) Ref   Richelson E. The use of cultured cells in the study of mood-normalizing drugs. Pharmacol. Toxicol. 66 suppl.3: 69-75, 1990. PubMed: 2179933adenosine; angiotensin II; bradykinin; enkephalin; glucagon; histamine H1; 5-hydroxytryptamine (serotonin, 5HT3); neurotensin; prostaglandin E; somatostatin; thrombin Ref Richelson E. The use of cultured cells in the study of mood-normalizing drugs. Pharmacol. Toxicol. 66 suppl.3: 69-75, 1990. PubMed: 2179933 |
| Comments： | The clone N1E was subcloned by isolation of single cells on glass shards. The adrenergic clone N1E-115 exhibits high levels of activity of the enzymes tyrosine hydroxylase and acetylcholinesterase which are necessary for neurotransmitter synthesis but are almost devoid of choline acetyltransferase. These cells contain 14 receptors for neurotransmitters.
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| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose (without sodium pyruvate), 90%; fetal bovine serum, 10%
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| Subculturing： |
Medium Renewal: Every 2 to 3 days.
Note: If DMEM formulation contain 1.5 g/L sodium bicarbonate. |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Time： | 36 hrs |
| Name of Depositor： | E Richelson |
| Deposited As： | Mus musculus |
| References： | . Methods in neurotransmitter receptor analysis. New York: Raven Press; 1990. Richelson E. The use of cultured cells in the study of mood-normalizing drugs. Pharmacol. Toxicol. 66 suppl.3: 69-75, 1990. PubMed: 2179933 Amano T, et al. Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase- acetylcholinesterase-tyrosine hydroxylase-axons-dendrites). Proc. Natl. Acad. Sci. USA 69: 258-263, 1972. PubMed: 4400294 Richelson E. Regulation of tyrosine hydroxylase activity in mouse neuroblastoma clone N1E-115. J. Neurochem. 21: 1139-1145, 1973. PubMed: 4148612 |