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| 产品名称： | MX |
|---|---|
| 商品货号： | TS211408 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | NOTE: this is a mixture of hybridoma cell lines from which the constituent individual cell lines must be separated by standard cloning and screening techniques. The plasmids, with inserts coding for the p21 deletion mutants corresponding to the hybridoma specificities, are also available as a mixed deposit, ATCC 67170. The hybridoma and plasmid specificities are as follows: HYBRIDOMAS: M38 = residues 1 - 23, M8 = residues 24 - 69, M70 = residues 90 - 106, M90 = residues 107 - 130, M30 = residues 131 - 152. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against several regions of the Harvey ras p21 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against several regions of the Harvey ras p21 |
| Comments： | Animals were immunized with Harvey ras p21. Spleen cells were fused with P3X63Ag8.653 myeloma cells. NOTE: this is a mixture of hybridoma cell lines from which the constituent individual cell lines must be separated by standard cloning and screening techniques. The plasmids, with inserts coding for the p21 deletion mutants corresponding to the hybridoma specificities, are also available as a mixed deposit, ATCC 67170. The hybridoma and plasmid specificities are as follows: HYBRIDOMAS: M38 = residues 1 - 23, M8 = residues 24 - 69, M70 = residues 90 - 106, M90 = residues 107 - 130, M30 = residues 131 - 152. PLASMIDS: p20 = deletion of residues 6 - 14, p19 = deletion of residues 6 - 22, p18 = deletion of residues 166 - 184, p17 = deletion of residues 153 - 184. p14 = deletion of residues 131 - 184, p12 = deletion of residues 107 - 184, p10 = deletion of residues 90 - 184, p8 = deletion of residues 70 - 184. |
| Complete Growth Medium： | Culture Medium: Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor： | The United States of America |
| U.S. Patent Number： |