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微信小章| 产品名称： | MTC-M |
|---|---|
| 商品货号： | TS211416 |
| Organism： | Mus musculus, mouse |
| Tissue： | thyroid |
| Cell Type： | C cell |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | 20 months |
| Gender： | female |
| Strain： | BALB/c |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from a transplantable neoplasm of thyroid C cells. |
| Clinical Data： | female |
| Genes Expressed： | somatostatin; calcitonin |
| Cellular Products： | somatostatin; calcitonin |
| Comments： | The cells can be propagated in spinner cultures or in static cultures. The amounts of stored and released somatostatin can be increased 2 to 3 fold by treatment for 4 days with 1 micromolar 5-bromo-2-deoxyuridine. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; heat-inactivated horse serum, 10%; fetal bovine serum, 5%
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 3 x 106 cells/mL. Cell clumps should be dispersed using a small bore pipette.
Medium Renewal:xa0Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Population Doubling Time： | 96 hrs |
| Name of Depositor： | AS Tischler |
| Deposited As： | Mus musculus |
| References： | Tischler AS, et al. Establishment of a continuous somatostatin-producing line of medullary thyroid carcinoma cells from BALB/c mice. J. Endocrinol. 110: 309-313, 1986. PubMed: 2875121 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |