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微信小章| 产品名称： | MPC-11 |
|---|---|
| 商品货号： | TS211418 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Storage Conditions： | liquid nitrogen vapor phase |
| Antigen Expression： | H-2d |
| Genes Expressed： | immunoglobulin; interleukin-6 (interleukin 6, IL-6) |
| Cellular Products： | immunoglobulin; interleukin-6 (interleukin 6, IL-6) |
| Virus Susceptibility： | Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana) |
| Virus Resistance： | {265A7263-515F-42F9-89FD-9B140DF21BEC} |
| Comments： | The cells contain A type viral particles. MPC-11 cells have been reported to produce murine IL-6. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Isotype： | IgG2b |
| Population Doubling Time： | 17 hrs |
| Name of Depositor： | MD Scharff |
| Deposited As： | Mus musculus |
| References： | Laskov R, et al. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. II. Assembly of IgG2b immunoglobulin by MPC 11 tumor and culture cells. J. Mol. Biol. 56: 327-339, 1971. PubMed: 5102530 Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J. Exp. Med. 131: 515-541, 1970. PubMed: 4189836 Coffino P, et al. Immunoglobulin production: method for quantitatively detecting variant myeloma cells. Science 167: 186-188, 1970. PubMed: 4188146 Blankenstein T, et al. DNA rearrangement and constitutive expression of the interleukin 6 gene in a mouse plasmacytoma. J. Exp. Med. 171: 965-970, 1990. PubMed: 2106569 Potter M, Kuff EL. Disorders in the differentiation of protein secretion in neoplastic plasma cells. J. Mol. Biol. 9: 537-544, 1964. PubMed: 14202284 |