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| 产品名称： | MRC-9 |
|---|---|
| 商品货号： | TS211422 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 15 weeks gestation |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | normal female; diploid; stable |
| Clinical Data： | 15 weeks gestation Caucasian female |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Yes, in semisolid medium |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 8,11 D16S539: 12 D5S818: 12,13 D7S820: 10,11 THO1: 6,8 TPOX: 11 vWA: 16,17 |
| Population Doubling Capacity： | 44 PDL |
| Name of Depositor： | JP Jacobs |
| Deposited As： | Homo sapiens |
| Year of Origin： | May, 1974 |
| References： | Jacobs JP, et al. Characteristics of a serially propagated human diploid cell designated MRC-9. J. Biol. Stand. 7: 113-122, 1979. PubMed: 113411 |