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微信小章| 产品名称： | MSTO-211H |
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| 商品货号： | TS211426 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | biphasic mesothelioma |
| Age： | 62 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 72; range = 70 to 78 |
| Derivation： | The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic mesothelioma of the lung. Derived from metastatic site, lung |
| Clinical Data： | The patient had not received prior radiation or chemotherapy. 62 years Caucasian male The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic mesothelioma of the lung. |
| Oncogene： | c-myc +; v-src +; v-abl +; v-erb B +; c-raf 1 +; H-ras +; K-ras +; N-ras +; N-myc -; L-myc -; c-myb -; c-fos -; v-fes -; v-fms -; v-sis - |
| Tumorigenic： | Yes |
| Effects： | Yes, tumors for med in approximately 20% of nude mice inoculated with MSTO-211H cells |
| Comments： | The patient had not received prior radiation or chemotherapy. MSTO-211H cells have high affinity binding sites for EGF, and express Neuron specific enolase (NSE) and both the alpha and beta subunits of human chorionic gonadotropin (HCG). L-DOPA decarboxylase (DDC), bombesin and neurotensin were not detected.
The cells overexpress the c-myc protooncogene, and no gene rearrangement or amplification was observed. They are positive for the expression of v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, and N-ras.
Expression of N-myc, L-myc, c-myb, c-fos, v-fes, v-fms and v-sis oncogene expression was not detected.
The cells can reach a saturation density of 400000 cells per sq cm, but will slough off of the surface as they attain this density.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11,14 D16S539: 13 D5S818: 12 D7S820: 8,12 THO1: 8,9.3 TPOX: 11 vWA: 16,18 |
| Population Doubling Time： | 20 hrs |
| Name of Depositor： | G Bepler |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1985 |
| References： | Bepler G, Koehler A. Multiple chromosomal aberrations and 11p allelotyping in lung cancer cell lines. Cancer Genet. Cytogenet. 84: 39-45, 1995. PubMed: 7497441 Bepler G, et al. Characterization of the state of differentiation of six newly established human non-small-cell lung cancer cell lines. Differentiation 37: 158-171, 1988. PubMed: 2840315 Haeder M, et al. Epidermal growth factor receptor expression in human lung cancer cell lines. Cancer Res. 48: 1132-1136, 1988. PubMed: 2830015 Lung Cancer 4: 155-161, 1988. |