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| 产品名称： | MNNG/HOS Cl #5 R-1059-D |
|---|---|
| 商品货号： | TS211427 |
| Organism： | Homo sapiens, human |
| Tissue： | bone |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | osteosarcoma |
| Age： | 13 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from HOS (ATCC CRL-1543) by transformation with 0.01 mcg/ml MNNG (a carcinogenic nitrosamine). |
| Clinical Data： | female Caucasian 13 years |
| Tumorigenic： | Yes |
| Effects： | Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments： | The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 2 to 5 days.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 8,11 vWA: 18 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | JS Rhim |
| Deposited As： | Homo sapiens |
| References： | Rhim JS, et al. Transformation of human cells in culture by N-methyl-N-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046 Rhim JS, et al. Characterization of human cells transformed in vitro by N-methyl-N- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298 Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224 |