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微信小章| 产品名称： | MOLT-4 |
|---|---|
| 商品货号： | TS211431 |
| Organism： | Homo sapiens, human |
| Cell Type： | T lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute lymphoblastic leukemia |
| Age： | 19 years |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a human cell line with the hypertetraploid chromosome number. The modal chromosome number was 95 occurring in 24% of cells. The rate of cells with higher ploidies was 0.8%. The 6q- and t(7;7) were paired and also common to all cells. The i(17q) was single and occurred only in some cells. Most normal chromosomes had four copies per cell except for the paired N7, X and Y, and two to three copies for N17. |
| Images： | 
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| Derivation： | MOLT-4 was derived from the same patient as the MOLT-3 cell line (ATCC CRL-1552). The line was established from cells taken from a patient in relapse. |
| Clinical Data： | male
19 years |
| Antigen Expression： | CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%) |
| Genes Expressed： | high levels of terminal deoxynucleotidyl transferase (TdT) are produced. |
| Cellular Products： | high levels of terminal deoxynucleotidyl transferase (TdT) are produced |
| Tumorigenic： | Yes |
| Effects： | Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice Ref   Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 |
| Comments： | The patient had received prior multidrug chemotherapy. There is a G -> A mutation at codon 248 of the p53 gene. P53 is not expressed. The cells do not produce immunoglobulin or Epstein-Barr virus. The line was originally contaminated with mycoplasma but has been cured with antibiotics. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 105 cells/mL and subculture before the cell density reaches 2 X 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: XY CSF1PO: 11, 12, 13 D13S317: 12, 13 D16S539: 11, 14 D5S818: 12 D7S820: 8, 10, 11 THO1: 6, 8 TPOX: 8 vWA: 17, 18 |
| Name of Depositor： | J Minowada |
| Deposited As： | Homo sapiens |
| References： | Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231 Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897 Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228 Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323 |
| Cross References： | Nucleotide (GenBank) : AF146433 Homo sapiens cell-line MOLT-4 sodium/hydrogen exchanger isoform 1 mRNA, complete cds. |