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微信小章| 产品名称： | Mo Mo T |
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| 商品货号： | TS211432 |
| Organism： | Homo sapiens, human |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 xa0Cells contain Retrovirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hairy cell leukemia |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Clinical Data： | male |
| Genes Expressed： | granulocyte macrophage colony stimulating factor (GM-CSF, GM CSF), also known as neutrophil migration inhibitory factor (NIF-T); interferon |
| Cellular Products： | granulocyte macrophage colony stimulating factor (GM-CSF, GM CSF), also known as neutrophil migration inhibitory factor (NIF-T); interferon |
| Comments： | A plasmid containing the DNA sequence coding from Mo that codes for CSF is available as ATCC 39754. Mo cells contain a replication competent genome of Human T Cell Leukemia Virus II (HTLV-II) and two defective HTLV-II genomes. For long term culture, the cells can be grown in medium with 2% fetal bovine serum. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | The cells are subcultured every 7 days by centrifugation and division of the cell pellet 1:4 (1:6 maximum). Do not dilute below 2 X 105 viable cells/ml or allow to exceed 2 X 106 cells/ml. Culture at 5% CO2.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 Note:The donor suggests using Falcon flasks for optimum growth. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C
Atmosphere: 8 to 9% CO2 in air |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10 D13S317: 9,13 D16S539: 11 D5S818: 11,12 D7S820: 9,12 THO1: 9.3 TPOX: 8,11 vWA: 17,18 |
| Name of Depositor： | Genetics Institute, Inc., Regents of the University of California |
| U.S. Patent Number： | |
| References： | Golde DW, Quan SG. Unique T-lymphocyte line and products derived therefrom. US Patent 4,438,032 dated Mar 20 1984 Clark SC, et al. Method for identification and isolation of DNA encoding a desired protein. US Patent 4,675,285 dated Jun 23 1987 Saxon A, et al. Immunologic characterization of hairy cell leukemias in continuous culture. J. Immunol. 120: 777-782, 1978. PubMed: 344797 Gasson JC, et al. Purified human granulocyte-macrophage colony-stimulating factor: direct action on neutrophils. Science 226: 1339-1342, 1984. PubMed: 6390681 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |